GMP-CKS-HE007-50μg / 询价
GMP-CKS-HE007-50μgx10 / 询价
GMP-CKS-HE007-50μgx20 / 询价
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GMP Human IL-7
Recombinant GMP Human IL-7 is expressed from E.coli without tag.It contains Asp26-His177 [Accession | P13232].
The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result.
≥ 95% as determined by SEC-HPLC.
≤ 0.01 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
The product is shipped with ice bag. Store it immediately at -20°C±5°C upon arrival.
-20°C±5°C for 36 months in lyophilized state.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
GMP Human IL-7 on Bis-Tris PAGE under reduced condition.
Measured in a cell proliferation assay using PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of GMP Human IL-7 is > 1 × 10^8 IU/mg, which is calibrated against the human IL-7 WHO reference standard (NIBSC code: 90/530) (QC Test).
KACTUS GMP grade cytokines are manufactured and tested in accordance with relevant GMP guidelines and regulatory requirements, and are in compliance with ISO 13485:2016 certified quality system.
● Personnel training programs
● Clean rooms and facility
● Facility maintenance and equipment calibration & verification
● Raw material testing and supplier evaluation and approval
● Animal-free manufacturing
● Rigorous quality control
● Stability testing(freeze-thaw and accelerated stability of reconstituted protein; long-term stability in lyophilized state)
● Comprehensive support files, including COA, COO, MSDS, TSE/BSE statement, etc.
We conduct comprehensive quality control testing to ensure that KACTUS GMP grade cytokines meet strict standards for identification, purity, safety, bioactivity and batch-to-batch consistency.
● Identification by Bis-Tris PAGE
● Purity by SEC-HPLC
● Endotoxin level
● Residual host cell protein
● Residual host cell DNA
● Mycoplasma testing
● Sterility testing
● Bioactivity (Specific activity against standards of WHO if applicable)
● Batch-to-batch consistency
Interleukin (IL)-7 is a non-redundant cytokine in T-cell development and function. It is required in early T-cell development as well as for T-cell homeostasis and is secreted by stromal cells in the thymic and bone marrow (BM) environment. IL-7 binds to its receptor which is composed of the two chains IL-7Rα (CD127), shared with the thymic stromal lymphopoietin (TSLP), and the common γ chain (CD132) for IL-2, IL-15, IL-9 and IL-21. Whereas γc is expressed by most hematopoietic cells, IL-7Rα is nearly exclusively expressed on lymphoid cells. After binding to its receptor, IL-7 signals through two different pathways: Jak-Stat (Janus kinase-Signal transducer and activator of transcription) and PI3K/Akt responsible for differentiation and survival, respectively.
IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
ElKassar N, Gress RE. An overview of IL-7 biology and its use in immunotherapy. J Immunotoxicol. 2010;7(1):1-7. doi:10.3109/15476910903453296
KACTUS GMP Human IL-7 is for research or ex vivo cell processing use, not for human or animal in vivo application.
